Solid phase phosphotriester synthesis of large oligodeoxyribonucleotides on a polyamide support
نویسندگان
چکیده
منابع مشابه
Rapid synthesis of oligodeoxyribonucleotides. IV. Improved solid phase synthesis of oligodeoxyribonucleotides through phosphotriester intermediates.
A phosphotriester solid phase method on a polyamide support has been used to prepare oligodeoxyribonucleotides up to 12 units long. Compared to solid phase phosphodiester synthesis the new methodology is quicker, more flexible and gives 10-60-fold better overall yields.
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A rapid solid phase method of oligonucleotide synthesis based on monomeric protected nucleosides has been developed.
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A study has been made of the kinetics of condensation reactions in phosphotriester synthesis of oligonucleotides on polystyrene copolymer resin support, using monomers, dimers, and trimers as the coupling units. The condensations follow pseudo first order kinetics, with a rate constant that decreases from monomers to dimers to trimers. Arrhenius plots indicate a nearly constant activation energ...
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Rapid synthesis of oligodeoxyribonucleotides is described by a solid phase method. Polysterene grafted on the surface of polytetrafluoroethylene has been found to be an adequate support. The properties of the support were studied in the synthesis of a number of oligonucleotides 6 to 15 nucleotides long. A flow-type semi-automatic synthesizer has been used. Each nucleotide addition involves seve...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1980
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/8.22.5193